SKP1 antibody (Middle Region)

Catalog No. ABIN357264

This anti-SKP1 antibody is a Rabbit Polyclonal antibody detecting SKP1 in WB and EIA. Suitable for Human and Mouse.

Quick Overview for SKP1 antibody (Middle Region) (ABIN357264)

Target: SKP1 (S-Phase Kinase-Associated Protein 1 (SKP1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SKP1 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-SKP1 Antibody

Binding Specificity: Middle Region

Specificity: This antibody detects SKP1 at center.

Purification: Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the central region of human SKP1A.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA 1: 1,000. Western blot 1: 100 - 1: 500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.

Target Details for SKP1

Target: SKP1 (S-Phase Kinase-Associated Protein 1 (SKP1))

Alternative Name: SKP1 / OCP2

Background: SKP1A is an F-box protein which functions as a substrate recognition component of the SCF ubiquitin ligase complex. It binds to cyclin F, S-phase kinase-associated protein 2, and other regulatory proteins involved in ubiquitin proteolysis through an F-box motif. SKP1A also collaborates with a network of proteins to control beta-catenin levels and affects the activity level of beta-catenin dependent TCF transcription factors. Studies have also characterized the protein as an RNA polymerase II elongation factor.Synonyms: Cyclin A/CDK2-associated protein p19, EMC19, OCP-2, OCP-II, Organ of Corti protein 2, RNA polymerase II elongation factor-like protein, S-phase kinase-associated protein 1, SKP1A, TCEB1L, Transcription elongation factor B, p19A, p19skp1

Molecular Weight: 18527 Da

Gene ID: 6500, 9606

UniProt: P63208

Pathways: Cell Division Cycle, Hedgehog Signaling, Mitotic G1-G1/S Phases