Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

EIF4E antibody (N-Term)

This anti-EIF4E antibody is a Rabbit Polyclonal antibody detecting EIF4E in WB, IHC (p) and EIA. Suitable for Human, Rat, Mouse and Zebrafish (Danio rerio).
Catalog No. ABIN357297

Quick Overview for EIF4E antibody (N-Term) (ABIN357297)

Target

See all EIF4E Antibodies
EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))

Reactivity

  • 124
  • 62
  • 50
  • 18
  • 5
  • 5
  • 5
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
Human, Rat, Mouse, Zebrafish (Danio rerio)

Host

  • 108
  • 15
  • 2
Rabbit

Clonality

  • 98
  • 27
Polyclonal

Conjugate

  • 59
  • 9
  • 8
  • 6
  • 5
  • 4
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
This EIF4E antibody is un-conjugated

Application

  • 98
  • 52
  • 44
  • 28
  • 27
  • 26
  • 16
  • 16
  • 15
  • 14
  • 7
  • 6
  • 1
  • 1
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
  • Binding Specificity

    • 40
    • 15
    • 8
    • 7
    • 6
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    N-Term

    Specificity

    This antibody detects EIF4E at N-term.

    Purification

    Protein G column, eluted with high and low pH buffers and neutralized immediately

    Immunogen

    This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human EIF4E Antibody.

    Isotype

    Ig Fraction
  • Application Notes

    ELISA 1: 1,000. Western blot 1: 250 - 1: 500. Immunohistochemistry 1: 50 - 1: 100.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    0.25 mg/mL

    Buffer

    PBS with 0.09 % (W/V) sodium azide

    Preservative

    Sodium azide

    Precaution of Use

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Avoid repeated freezing and thawing.

    Storage

    4 °C/-20 °C

    Storage Comment

    Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.
  • Target

    EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))

    Alternative Name

    EIF4E

    Background

    EIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G1/EIF4G3. EIF4E is also known to interact with other partners. The interaction with EIF4ENIF1 mediates the import into the nucleus. Nonphosphorylated EIF4EBP1, EIF4EBP2 and EIF4EBP3 compete with EIF4G1/EIF4G3 to interact with EIF4E, insulin stimulated MAP-kinase (MAPK1 and MAPK3) phosphorylation of EIF4EBP1 causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and consequent initiation of translation. Rapamycin can attenuate insulin stimulation, mediated by FKBPs. this gene also interacts mutually exclusive with EIF4A1 and EIF4A2.Synonyms: EIF-4E, EIF4EL1, EIF4F, Eukaryotic translation initiation factor 4E, eIF-4F 25 kDa subunit, mRNA cap-binding protein

    Molecular Weight

    25097 Da

    Gene ID

    1977, 9606

    UniProt

    P06730

    Pathways

    BCR Signaling
You are here:
Chat with us!