NCAPH2 antibody (N-Term)

Catalog No. ABIN357333

This anti-NCAPH2 antibody is a Rabbit Polyclonal antibody detecting NCAPH2 in WB and EIA. Suitable for Human.

Quick Overview for NCAPH2 antibody (N-Term) (ABIN357333)

Target: NCAPH2 (Non-SMC Condensin II Complex, Subunit H2 (NCAPH2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NCAPH2 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-NCAPH2 Antibody

Binding Specificity: N-Term

Purification: Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human NCAPH2.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA 1: 1,000. Western blot 1: 100 - 1: 500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.

Target Details for NCAPH2

Target: NCAPH2 (Non-SMC Condensin II Complex, Subunit H2 (NCAPH2))

Alternative Name: NCAPH2

Background: Structural maintenance of chromosomes (SMC) and non-SMC condensin proteins associate into complexes that have been implicated in the process of chromosome condensation. A crucial prerequisite for accurate segregation of replicated sister chromatids is the condensation of the chromosomes into a manageable form prior to metaphase. The condensin I complex consists of two SMC subunits, SMC2 and SMC4, and three non-SMC subunits, CAP-H, CAP-G, and CAP-D2. An alternative complex, the condensin II complex, contains alternate non-SMC subunits, CAP-G2, CAP-H2, and CAP-D3. CAP-H2 is also known as Non-SMC condensin II complex, subunit H2 (NCAPH2) or kleisin beta isoform 2. The three non-SMC subunits in the condensing complexes form a regulatory subcomplex that is required to activate the SMC ATPases and to promote mitosis-specific chromatin binding of the holocomplex. The precise individual functions of each non-SMC protein in activation remain to be determined.Synonyms: CAPH2, Chromosome-associated protein H2

Molecular Weight: 62463 Da

Gene ID: 29781

NCBI Accession: NP_001171940

UniProt: Q6IBW4