UBE2D1 antibody (C-Term)

Catalog No. ABIN357485

The Rabbit Polyclonal anti-UBE2D1 antibody has been validated for WB, IHC (p) and EIA. It is suitable to detect UBE2D1 in samples from Human and Mouse.

Quick Overview for UBE2D1 antibody (C-Term) (ABIN357485)

Target: UBE2D1 (Ubiquitin-Conjugating Enzyme E2D 1 (UBE2D1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBE2D1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-UBE2D1 Antibody

Binding Specificity: C-Term

Specificity: This antibody is specific to UBE2D1/SFT (C-term).

Purification: Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human UBE2D1.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA: 1/1,000. Western Blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for UBE2D1

Target: UBE2D1 (Ubiquitin-Conjugating Enzyme E2D 1 (UBE2D1))

Alternative Name: UBE2D1 / SFT

Background: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2D1 is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is closely related to a stimulator of iron transport (SFT), and is up-regulated in hereditary hemochromatosis. It also functions in the ubiquitination of the tumor-suppressor protein p53 and the hypoxia-inducible transcription factor HIF1alpha by interacting with the E1 ubiquitin-activating enzyme and the E3 ubiquitin-protein ligases.Synonyms: E2(17)KB 1, UBC4/5 homolog, UBCH5, UBCH5A, Ubiquitin carrier protein D1, Ubiquitin-conjugating enzyme E2 D1, Ubiquitin-protein ligase D1

Molecular Weight: 16602 Da

Gene ID: 7321, 5874

UniProt: P51668

Pathways: Activation of Innate immune Response, Toll-Like Receptors Cascades