UBE2E3 antibody (C-Term)

Catalog No. ABIN357599

The Rabbit Polyclonal anti-UBE2E3 antibody has been validated for WB and EIA. It is suitable to detect UBE2E3 in samples from Human.

Quick Overview for UBE2E3 antibody (C-Term) (ABIN357599)

Target: UBE2E3 (Ubiquitin-Conjugating Enzyme E2E 3 (UBE2E3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBE2E3 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-UBE2E3 Antibody

Binding Specificity: C-Term

Specificity: This antibody is specific to UBCH9/UBE2E3 (C-term). Predicted to cross react with Mouse (100 % Antigen Homology).

Purification: Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human UBCH9.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA: 1/1,000. Western Blot: 1/100-1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for UBE2E3

Target: UBE2E3 (Ubiquitin-Conjugating Enzyme E2E 3 (UBE2E3))

Alternative Name: UBE2E3

Background: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBCH9 is a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein shares 100 % sequence identity with the mouse and rat counterparts, which indicates that this enzyme is highly conserved in eukaryotes. Two alternatively spliced transcript variants encoding the same protein have been found.Synonyms: UBCE4, UBCH9, UbcM2, Ubiquitin carrier protein E3, Ubiquitin-conjugating enzyme E2 E3, Ubiquitin-conjugating enzyme E2-23 kDa, Ubiquitin-protein ligase E3

Molecular Weight: 22912 Da

Gene ID: 10477, 9606

UniProt: Q969T4