PIGK antibody (N-Term)

Catalog No. ABIN357725

The Rabbit Polyclonal anti-PIGK antibody has been validated for WB, IHC (p) and EIA. It is suitable to detect PIGK in samples from Human.

Quick Overview for PIGK antibody (N-Term) (ABIN357725)

Target: PIGK (GPI8) (GPI-anchor transamidase (GPI8))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PIGK antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-PIGK Antibody

Binding Specificity: N-Term

Specificity: This antibody detects GPI8 (N-term). Predicted to cross react with Mouse (100 % Antigen Homology).

Purification: Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human GPI8.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA: 1/1,000. Western blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for PIGK

Target: PIGK (GPI8) (GPI-anchor transamidase (GPI8))

Alternative Name: PIGK

Background: GPI8 is a member of the cysteine protease family C13 that is involved in glycosylphosphatidylinositol (GPI)-anchor biosynthesis. The GPI-anchor is a glycolipid found on many blood cells and serves to anchor proteins to the cell surface. This protein is a member of the multisubunit enzyme, GPI transamidase and is thought to be its enzymatic component. GPI transamidase mediates GPI anchoring in the endoplasmic reticulum, by catalyzing the transfer of fully assembled GPI units to proteins.Synonyms: GPI transamidase, GPI-anchor transamidase, GPI8, PIG-K, Phosphatidylinositol-glycan biosynthesis class K protein

Molecular Weight: 45251 Da

Gene ID: 10026, 5874

UniProt: Q92643