Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

SAE1 antibody (C-Term)

This anti-SAE1 antibody is a Rabbit Polyclonal antibody detecting SAE1 in WB, IHC (p) and EIA. Suitable for Human and Mouse.
Catalog No. ABIN357744

Quick Overview for SAE1 antibody (C-Term) (ABIN357744)

Target

See all SAE1 Antibodies
SAE1 (SUMO1 Activating Enzyme Subunit 1 (SAE1))

Reactivity

  • 61
  • 40
  • 18
  • 5
  • 5
  • 4
  • 4
  • 4
  • 3
  • 3
  • 2
  • 1
  • 1
Human, Mouse

Host

  • 68
  • 7
  • 1
Rabbit

Clonality

  • 60
  • 16
Polyclonal

Conjugate

  • 43
  • 4
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This SAE1 antibody is un-conjugated

Application

  • 59
  • 26
  • 19
  • 19
  • 13
  • 13
  • 13
  • 9
  • 5
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
  • Binding Specificity

    • 15
    • 8
    • 6
    • 5
    • 5
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term

    Specificity

    This antibody is specific to SAE1/AOS1 (C-term).

    Purification

    Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

    Immunogen

    This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human AOS1.

    Isotype

    Ig Fraction
  • Application Notes

    ELISA: 1/1,000. Western Blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    0.25 mg/mL

    Buffer

    PBS with 0.09 % (W/V) Sodium Azide as preservative.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Avoid repeated freezing and thawing.

    Storage

    4 °C/-20 °C

    Storage Comment

    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
  • Target

    SAE1 (SUMO1 Activating Enzyme Subunit 1 (SAE1))

    Alternative Name

    SAE1 / AOS1

    Background

    The dimeric enzyme AOS1 acts as a an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins and formation of a thioester with a conserved cysteine residue on SAE2.Synonyms: SUA1, SUMO-activating enzyme subunit 1, UBLE1A, Ubiquitin-like 1-activating enzyme E1A

    Molecular Weight

    38450 Da

    Gene ID

    10055, 9606

    UniProt

    Q9UBE0
You are here:
Chat with us!