Crk antibody (pSer41)

Catalog No. ABIN358272

The Rabbit Polyclonal anti-Crk antibody (ABIN358272) specifically detects Crk in EIA. The antibody is reactive with Human samples.

Quick Overview for Crk antibody (pSer41) (ABIN358272)

Target: Crk (CRK) (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian) (CRK))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Crk antibody is un-conjugated

Application: Enzyme Immunoassay (EIA)

Product Details anti-Crk Antibody

Binding Specificity: pSer41

Specificity: This antibody detects CRK pSer41. Predicted to cross react with Mouse (100 % Antigen Homology).

Purification: Protein A Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with peptides. The antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S41 of human CRK.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA: 1/1,000. Dot Blot: 1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for Crk

Target: Crk (CRK) (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian) (CRK))

Alternative Name: CRK

Background: CRK is a member of an adapter protein family that binds to several tyrosine-phosphorylated proteins. It has several SH2 and SH3 domains (src-homology domains) and is involved in several signaling pathways, recruiting cytoplasmic proteins in the vicinity of tyrosine kinase through SH2-phosphotyrosine interaction. The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation.Synonyms: Adapter molecule crk, Proto-oncogene C-crk, p38

Molecular Weight: 22906 Da

Gene ID: 1398

NCBI Accession: NP_005197

UniProt: P46108

Pathways: Neurotrophin Signaling Pathway, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor