GIT1 antibody (pTyr554)

Catalog No. ABIN358481

The Rabbit Polyclonal anti-GIT1 antibody has been validated for EIA. It is suitable to detect GIT1 in samples from Human.

Quick Overview for GIT1 antibody (pTyr554) (ABIN358481)

Target: GIT1

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GIT1 antibody is un-conjugated

Application: Enzyme Immunoassay (EIA)

Product Details anti-GIT1 Antibody

Binding Specificity: pTyr554

Specificity: This antibody detects GIT1 pTyr554. Predicted to cross react with Mouse (100 % Antigen Homology).

Purification: Protein A Chromatography followed by two-step phosphospecific peptide affinity purification.

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S554 of human GIT1.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA: 1/1,000. Dot Blot: 1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for GIT1

Target: GIT1

Alternative Name: GIT1

Background: GIT1 is a GTPase-activating protein for the ADP ribosylation factor family. It may serve as a scaffold to bring together molecules to form signaling modules controlling vesicle trafficking, adhesion and cytoskeletal organization, increases the speed of cell migration, as well as the size and rate of formation of protrusions, possibly by targeting PAK1 to adhesions and the leading edge of lamellipodia. It sequesters inactive non-tyrosine-phosphorylated paxillin in cytoplasmic complexes.Synonyms: ARF GTPase-activating protein GIT1, CAT1, Cool-associated and tyrosine-phosphorylated protein 1, G protein-coupled receptor kinase-interactor 1, GRK-interacting protein 1

Molecular Weight: 84341 Da

Gene ID: 28964, 9606

UniProt: Q9Y2X7