GREM1 antibody (C-Term)

Catalog No. ABIN358612

This Rabbit Polyclonal antibody specifically detects GREM1 in WB, IHC (p) and EIA. It exhibits reactivity toward Human and Mouse.

Quick Overview for GREM1 antibody (C-Term) (ABIN358612)

Target: GREM1 (Gremlin 1 (GREM1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GREM1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-GREM1 Antibody

Binding Specificity: C-Term

Specificity: This antibody reacts to GREMLIN.

Purification: Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminus of human Gremlin.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA: 1/1,000. Western blotting: 1/100 - 1/500. Immunohistochemistry (Application validated by third-party citation).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for GREM1

Target: GREM1 (Gremlin 1 (GREM1))

Alternative Name: Gremlin-1 / GREM1

Background: GREMLIN is a member of the BMP (bone morphogenic protein) antagonist family. Like BMPs, BMP antagonists contain cystine knots and typically form homo- and heterodimers. The CAN (cerberus and dan) subfamily of BMP antagonists, to which this gene belongs, is characterized by a C-terminal cystine knot with an eight-membered ring. The antagonistic effect of the secreted glycosylated protein encoded by this gene is likely due to its direct binding to BMP proteins. As an antagonist of BMP, this gene may play a role in regulating organogenesis, body patterning, and tissue differentiation. In mouse, this protein has been shown to relay the sonic hedgehog (SHH) signal from the polarizing region to the apical ectodermal ridge during limb bud outgrowth.Synonyms: CKTSF1B1, Cell proliferation-inducing gene 2 protein, Cysteine knot superfamily 1 BMP antagonist 1, DAN domain family member 2, DAND2, DRM, GREM-1, IHG-2, PIG2

Gene ID: 26585

NCBI Accession: NP_037504

UniProt: O60565

Pathways: Regulation of Muscle Cell Differentiation, Tube Formation, Maintenance of Protein Location