Claudin 1 antibody (Loop 1)

Catalog No. ABIN358846

This Rabbit Polyclonal antibody specifically detects Claudin 1 in WB, IHC (p) and EIA. It exhibits reactivity toward Human.

Quick Overview for Claudin 1 antibody (Loop 1) (ABIN358846)

Target: Claudin 1 (CLDN1)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Claudin 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-Claudin 1 Antibody

Binding Specificity: Loop 1

Specificity: This antibody reacts to Claudin 1 (CLDN1).

Purification: Protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the Loop1 region of human CLDN1.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA: 1/1,000. Western blotting: 1/50 - 1/100. Immunohistochemistry: 1/10 - 1/50.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for Claudin 1

Target: Claudin 1 (CLDN1)

Alternative Name: Claudin-1 / CLDN1

Background: Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. CLDN1, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. Loss of function mutations result in neonatal ichthyosis-sclerosing cholangitis syndrome.Synonyms: CLD1, PRO944, SEMP1, Senescence-associated epithelial membrane protein, UNQ481

Gene ID: 9076, 9606

UniProt: O95832

Pathways: Cell-Cell Junction Organization, Hepatitis C