MAP4K2 antibody (Middle Region)

Catalog No. ABIN359142

This anti-MAP4K2 antibody is a Rabbit Polyclonal antibody detecting MAP4K2 in WB, IHC (p) and EIA. Suitable for Human and Mouse.

Quick Overview for MAP4K2 antibody (Middle Region) (ABIN359142)

Target: MAP4K2 (Mitogen-Activated Protein Kinase Kinase Kinase Kinase 2 (MAP4K2))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAP4K2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-MAP4K2 Antibody

Binding Specificity: Middle Region

Specificity: This antibody reacts to Germinal Center Kinase (GCK/MAP4K2).

Purification: Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the center region of human MAP4K2.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA: 1/1,000. Western blotting: 1/100 - 1/500. Immunohistochemistry: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for MAP4K2

Target: MAP4K2 (Mitogen-Activated Protein Kinase Kinase Kinase Kinase 2 (MAP4K2))

Alternative Name: MAP4K2

Background: The protein encoded by this gene is a member of the serine/threonine protein kinase family. Although this kinase is found in many tissues, its expression in lymphoid follicles is restricted to the cells of germinal centre, where it may participate in B-cell differentiation. This kinase can be activated by TNF-alpha, and has been shown to specifically activate MAP kinases. This kinase is also found to interact with TNF receptor-associated factor 2 (TRAF2), which is involved in the activation of MAP3K1/MEKK1.Synonyms: B lymphocyte serine/threonine-protein kinase, Germinal center kinase, MAPK, MEK kinase kinase 2, MEKKK 2, Mitogen-activated protein kinase kinase kinase kinase 2, RAB8IP, Rab8-interacting protein

Gene ID: 5871, 5874

UniProt: Q12851