EIF4A1 antibody (N-Term)

Catalog No. ABIN359486

The Rabbit Polyclonal anti-EIF4A1 antibody has been validated for WB, IHC (p) and EIA. It is suitable to detect EIF4A1 in samples from Human.

Quick Overview for EIF4A1 antibody (N-Term) (ABIN359486)

Target: EIF4A1 (Eukaryotic Translation Initiation Factor 4A1 (EIF4A1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF4A1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-EIF4A1 Antibody

Binding Specificity: AA 1-30, N-Term

Specificity: This antibody detects EIF4A1 at N-term.

Cross-Reactivity (Details): Species reactivity (expected):Bovine, Mouse, Monkey.
Species reactivity (tested):Human.

Purification: Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: KLH conjugated synthetic peptide peptide between 1~30 amino acids from the N-terminal region of Human EIF4A1. Genename: EIF4A1

Isotype: Ig Fraction

Application Details

Application Notes: ELISA: 1/1,000. Western blot: 1/50-1/100. Immunohistochemistry on Paraffin Sections: 1/10-1/50.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS, 0.09 % (W/V) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for EIF4A1

Target: EIF4A1 (Eukaryotic Translation Initiation Factor 4A1 (EIF4A1))

Alternative Name: EIF4A1

Background: EIF4A1 is an ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.Synonyms: ATP-dependent RNA helicase eIF4A-1, DDX2A, EIF4A, Eukaryotic initiation factor 4A-I, eIF-4A-I, eIF4A-I

Molecular Weight: 46154 Da (Calculated)

Gene ID: 1973, 9606

UniProt: P60842