PUM1 antibody (N-Term)

Catalog No. ABIN359732

The Rabbit Polyclonal anti-PUM1 antibody has been validated for WB, IHC (p) and EIA. It is suitable to detect PUM1 in samples from Human.

Quick Overview for PUM1 antibody (N-Term) (ABIN359732)

Target: PUM1 (Pumilio Homolog 1 (PUM1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PUM1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-PUM1 Antibody

Binding Specificity: N-Term

Specificity: This antibody reacts to Pumilio 1 (PUM1).

Purification: Protein A column, followed by peptide affinity purification

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human PUM1.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA: 1/1,000. Western blotting: 1/50 - 1/100. Immunohistochemistry: 1/10 - 1/50.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for PUM1

Target: PUM1 (Pumilio Homolog 1 (PUM1))

Alternative Name: Pumilio 1 / PUM1

Background: PUM1 is a member of the PUF family, evolutionarily conserved RNA-binding proteins related to the Pumilio proteins of Drosophila and the fem-3 mRNA binding factor proteins of C. elegans. This protein contains a sequence-specific RNA binding domain comprised of eight repeats and N- and C-terminal flanking regions, and serves as a translational regulator of specific mRNAs by binding to their 3' untranslated regions. The evolutionarily conserved function of this protein in invertebrates and lower vertebrates suggests that the human protein may be involved in translational regulation of embryogenesis, and cell development and differentiation.Synonyms: HsPUM, KIAA0099, PUMH1, Pumilio homolog 1, Pumilio-1

Gene ID: 9698, 9606

UniProt: Q14671