Cullin 5 antibody (C-Term)

Catalog No. ABIN359745

This anti-Cullin 5 antibody is a Rabbit Polyclonal antibody detecting Cullin 5 in WB and EIA. Suitable for Human.

Quick Overview for Cullin 5 antibody (C-Term) (ABIN359745)

Target: Cullin 5 (CUL5)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cullin 5 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-Cullin 5 Antibody

Binding Specificity: C-Term

Specificity: This antibody reacts with CUL5 (C-term).

Purification: Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human CUL5.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA: 1/1,000. Western Blot: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for Cullin 5

Target: Cullin 5 (CUL5)

Alternative Name: Cullin-5

Background: CUL5 is a core component of multiple SCF-like ECS (Elongin-Cullin 2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component. ECS(SOCS1) seems to direct ubiquitination of JAk2. It seems to be involved poteosomal degradation of p53/TP53 stimulated by adenovirus E1B-55 kDa protein and may form a cell surface vasopressin receptor.Synonyms: CUL-5, CUL5, VACM-1, VACM1, Vasopressin-activated calcium-mobilizing receptor

Molecular Weight: 90955 Da predicted from amino acid sequence. Theoretical pI: 8.08

Gene ID: 8065, 9606

UniProt: Q93034

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development