PUM2 antibody (Ser182)

Catalog No. ABIN360017

The Rabbit Polyclonal anti-PUM2 antibody is suitable to detect PUM2 in samples from Human. It has been validated for WB, FACS, IHC (p) and EIA.

Quick Overview for PUM2 antibody (Ser182) (ABIN360017)

Target: PUM2 (Pumilio Homolog 2 (Drosophila) (PUM2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PUM2 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-PUM2 Antibody

Binding Specificity: Ser182

Specificity: This antibody recognizes Pumilio 2 (PUM2).

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Protein A Chromatography followed by peptide affinity purification.

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding Ser182 of Human PUM2.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA: 1/1,000. Western blot 1/50approx. 1/100. Immunohistochemistry: 1/10approx. 1/50. Flow Cytometry: 1/10approx. 1/50.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for PUM2

Target: PUM2 (Pumilio Homolog 2 (Drosophila) (PUM2))

Alternative Name: Pumilio 2 / PUM2

Background: PUM2 is a sequence-specific RNA-binding protein that regulates translation and mRNA stability by binding the 3'-UTR of mRNA targets. Its interactions and tissue specificity suggest that it may be required to support proliferation and self-renewal of stem cells by regulating the translation of key transcripts.Synonyms: KIAA0235, PUM-2, PUMH2, Pumilio homolog 2, Pumilio-2

Molecular Weight: 114216 Da

Gene ID: 23369, 9606

UniProt: Q8TB72

Pathways: Ribonucleoprotein Complex Subunit Organization