The Rabbit Polyclonal anti-KLF10/TIEG1 antibody is suitable to detect KLF10/TIEG1 in samples from Human. It has been validated for WB, IHC (p) and EIA.
Prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Immunogen
This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 19~49 amino acids from the N-terminal region of Human TIEG.
KLF10
Reactivity: Human
WB, ELISA
Host: Mouse
Monoclonal
6D5
unconjugated
Application Notes
ELISA: 1/1,000. Western blotting: 1/50-1/100. Immunohistochemistry: 1/10-1/50. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Format
Liquid
Concentration
0.25 mg/mL
Buffer
PBS with 0.09 % (W/V) Sodium Azide as preservative.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
TIEG is a transcriptional repressor involved in the regulation of cell growth. TIEG binds to the consensus sequence 5'-GGTGTG-3'and inhibits cell growth.Synonyms: EGR-alpha, Krueppel-like factor 10, TGFB-inducible early growth response protein 1, TIEG, TIEG-1, Transforming growth factor-beta-inducible early growth response protein 1
Molecular Weight
52555 Da predicted by amino acid sequence. Theoretical pI: 9.29 Da