LIM Domain Kinase 1 antibody (N-Term)
Quick Overview for LIM Domain Kinase 1 antibody (N-Term) (ABIN360142)
Target
See all LIM Domain Kinase 1 (LIMK1) AntibodiesReactivity
Host
Clonality
Conjugate
Application
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Binding Specificity
- N-Term
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Specificity
- This antibody reacts to LIM Kinase 1 (LIMK1).
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Purification
- Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
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Immunogen
- This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human LIMK1.
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Isotype
- Ig Fraction
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Application Notes
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ELISA: 1/1,000. Western blotting: 1/100 - 1/500. Immunohistochemistry: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 0.25 mg/mL
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Buffer
- PBS with 0.09 % (W/V) sodium azide
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Preservative
- Sodium azide
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Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handling Advice
- Avoid repeated freezing and thawing.
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Storage
- 4 °C/-20 °C
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Storage Comment
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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- LIM Domain Kinase 1 (LIMK1)
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Alternative Name
- LIMK1
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Background
- LIMK1, a member of the Ser/Thr protein kinase family, may be a component of an intracellular signaling pathway and may be involved in brain development. It phosphorylates and inactivates the actin binding/depolymerizing factor cofilin and induces actin cytoskeletal changes. The LIM domain interacts with the cytoplasmic domain of NRG1, and this cytoplasmic protein also binds ROCK1, whic phosphorylates LIMK1 on serine and/or threonine residues. Highest expression occurs in both adult and fetal nervous systems. It is detected ubiquitously throughout the different regions of adult brain, with highest levels in the cerebral cortex, and is expressed to a lesser extent in heart and skeletal muscle. Haploinsufficiency of LIMK1 may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in Williams-Beuren syndrome (WBS), a rare developmental disorder. It is a contiguous gene deletion syndrome involving genes from chromosome band 7q11.23. This protein contains 2 LIM zinc-binding domains and 1 PDZ/DHR domain.Synonyms: EC=2.7.11.1, LIM domain kinase 1, LIMK, LIMK-1
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Gene ID
- 3984, 9606
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UniProt
- P53667
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Pathways
- Caspase Cascade in Apoptosis, Regulation of Cell Size, CXCR4-mediated Signaling Events
Target
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