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LIM Domain Kinase 1 antibody (N-Term)

This Rabbit Polyclonal antibody specifically detects LIM Domain Kinase 1 in WB, IHC (p) and EIA. It exhibits reactivity toward Human.
Catalog No. ABIN360142

Quick Overview for LIM Domain Kinase 1 antibody (N-Term) (ABIN360142)

Target

See all LIM Domain Kinase 1 (LIMK1) Antibodies
LIM Domain Kinase 1 (LIMK1)

Reactivity

  • 105
  • 79
  • 73
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
Human

Host

  • 101
  • 20
  • 3
Rabbit

Clonality

  • 104
  • 20
Polyclonal

Conjugate

  • 58
  • 9
  • 8
  • 5
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
This LIM Domain Kinase 1 antibody is un-conjugated

Application

  • 89
  • 49
  • 26
  • 26
  • 26
  • 17
  • 16
  • 8
  • 7
  • 6
  • 6
  • 3
  • 1
  • 1
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
  • Binding Specificity

    • 23
    • 15
    • 15
    • 8
    • 7
    • 7
    • 5
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    N-Term

    Specificity

    This antibody reacts to LIM Kinase 1 (LIMK1).

    Purification

    Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS

    Immunogen

    This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human LIMK1.

    Isotype

    Ig Fraction
  • Application Notes

    ELISA: 1/1,000. Western blotting: 1/100 - 1/500. Immunohistochemistry: 1/50 - 1/100.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    0.25 mg/mL

    Buffer

    PBS with 0.09 % (W/V) sodium azide

    Preservative

    Sodium azide

    Precaution of Use

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Avoid repeated freezing and thawing.

    Storage

    4 °C/-20 °C

    Storage Comment

    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
  • Target

    LIM Domain Kinase 1 (LIMK1)

    Alternative Name

    LIMK1

    Background

    LIMK1, a member of the Ser/Thr protein kinase family, may be a component of an intracellular signaling pathway and may be involved in brain development. It phosphorylates and inactivates the actin binding/depolymerizing factor cofilin and induces actin cytoskeletal changes. The LIM domain interacts with the cytoplasmic domain of NRG1, and this cytoplasmic protein also binds ROCK1, whic phosphorylates LIMK1 on serine and/or threonine residues. Highest expression occurs in both adult and fetal nervous systems. It is detected ubiquitously throughout the different regions of adult brain, with highest levels in the cerebral cortex, and is expressed to a lesser extent in heart and skeletal muscle. Haploinsufficiency of LIMK1 may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in Williams-Beuren syndrome (WBS), a rare developmental disorder. It is a contiguous gene deletion syndrome involving genes from chromosome band 7q11.23. This protein contains 2 LIM zinc-binding domains and 1 PDZ/DHR domain.Synonyms: EC=2.7.11.1, LIM domain kinase 1, LIMK, LIMK-1

    Gene ID

    3984, 9606

    UniProt

    P53667

    Pathways

    Caspase Cascade in Apoptosis, Regulation of Cell Size, CXCR4-mediated Signaling Events
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