PKN3 antibody (C-Term)

Catalog No. ABIN360331

This Rabbit Polyclonal antibody specifically detects PKN3 in WB, IHC (p) and EIA. It exhibits reactivity toward Human.

Quick Overview for PKN3 antibody (C-Term) (ABIN360331)

Target: PKN3 (Protein Kinase N3 (PKN3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PKN3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-PKN3 Antibody

Binding Specificity: C-Term

Specificity: This antibody reacts to PKN beta.

Purification: Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human PKN beta.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA: 1/1,000. Western blotting: 1/100 - 1/500. Immunohistochemistry: 1/10 - 1/50.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for PKN3

Target: PKN3 (Protein Kinase N3 (PKN3))

Alternative Name: PKN3

Background: PKN3, a protein kinase C-related molecule, mediates malignant cell growth downstream of activated PI3K. PKN3 is required for invasive prostate cell growth as assessed by 3D cell culture assays and in an orthotopic mouse tumor model by inducible expression of short hairpin RNA (shRNA). PKN3 is regulated by PI3K at both the expression level and the catalytic activity level. Therefore, PKN3 might represent a preferred target for therapeutic intervention in cancers that lack tumor suppressor PTEN function or depend on chronic activation of PI3K.Synonyms: PKN beta, PKN-beta, PKNBETA, Protein-kinase C-related kinase 3, Serine/threonine-protein kinase N3

Gene ID: 29941, 9606

UniProt: Q6P5Z2