DGKA antibody (C-Term)

Catalog No. ABIN360612

This anti-DGKA antibody is a Rabbit Polyclonal antibody detecting DGKA in WB, IHC (p) and EIA. Suitable for Human.

Quick Overview for DGKA antibody (C-Term) (ABIN360612)

Target: DGKA (Diacylglycerol Kinase, alpha 80kDa (DGKA))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DGKA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-DGKA Antibody

Binding Specificity: C-Term

Specificity: This antibody reacts to DGKA.

Purification: Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human DGKA.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA: 1/1,000. Western Blot: 1/50 - 1/200. Immunohistochemistry: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for DGKA

Target: DGKA (Diacylglycerol Kinase, alpha 80kDa (DGKA))

Alternative Name: DAG Kinase alpha

Background: Upon cell stimulation, the kinase DGKA converts the second messenger diacylglycerol into phophatidate, initiating the resynthesis of phosphatidylinositols and attenuating protein kinase C activity. DGKA is stimulated by calcium and phosphatidylserine, and is phosphorylated by protein kinase C. Tissue expression is in lymphocytes and oligodengroglial cells. DGKA contains 2 zinc-dependent phorbol-ester and DAG binding domains, and 2 EF-hand calcium binding domains.Synonyms: DAGK, DAGK1, DGK-alpha, DGKA, Diacylglycerol kinase alpha, Diglyceride kinase alpha

Gene ID: 1606, 9606

UniProt: P23743