Cytidine Monophosphate (UMP-CMP) Kinase 1, Cytosolic (CMPK1) (C-Term) antibody

Catalog No. ABIN360614

The Rabbit Polyclonal anti- antibody has been validated for WB, IHC (p) and EIA. It is suitable to detect in samples from Human and Mouse.

Quick Overview for Cytidine Monophosphate (UMP-CMP) Kinase 1, Cytosolic (CMPK1) (C-Term) antibody (ABIN360614)

Target: Cytidine Monophosphate (UMP-CMP) Kinase 1, Cytosolic (CMPK1)

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details

Binding Specificity: C-Term

Specificity: This antibody reacts to UCK.

Purification: Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human UCK.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA: 1/1,000. Western blotting: 1/100 - 1/500. Immunohistochemistry: 1/50 - 1/100. Flow cytometry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details

Target: Cytidine Monophosphate (UMP-CMP) Kinase 1, Cytosolic (CMPK1)

Alternative Name: CMPK1

Background: UCK1 (Uridine-cytidine kinase 1) phosphorylates uridine and cytidine to uridine monophosphate and cytidine monophosphate. This enzyme does not phosphorylate deoxyribonucleosides or purine ribonucleosides. Of note, UCK1 is able to use either ATP or GTP as a phosphate donor. UCK1 also possesses the ability to phosphorylate a number of cytidine and uridine nucleoside analogs such as 6-azauridine, 5-fluorouridine, 4-thiouridine, 5-bromouridine, N(4)-acetylcytidine, N(4)-benzoylcytidine, 5-fluorocytidine, 2-thiocytidine, 5-methylcytidine, and N(4)-anisoylcytidine.Synonyms: CMK, CMPK, Cytidine monophosphate kinase, Cytidylate kinase, Deoxycytidylate kinase, UCK, UMK, UMP-CMP kinase, UMP/CMP kinase, UMP/CMPK, UMPK, Uridine monophosphate kinase, Uridine monophosphate/cytidine monophosphate kinase

Gene ID: 51727, 9606

UniProt: P30085

Pathways: Nucleotide Phosphorylation, Ribonucleoside Biosynthetic Process