PPM1F antibody (N-Term)

Catalog No. ABIN360800

The Rabbit Polyclonal anti-PPM1F antibody has been validated for WB, IHC (p) and EIA. It is suitable to detect PPM1F in samples from Human.

Quick Overview for PPM1F antibody (N-Term) (ABIN360800)

Target: PPM1F (Protein Phosphatase 1F (PP2C Domain Containing) (PPM1F))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PPM1F antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-PPM1F Antibody

Binding Specificity: N-Term

Specificity: This antibody reacts to PPM1F.

Purification: Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human PPM1F.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA: 1/1,000. Western blotting: 1/100 - 1/500. Immunohistochemistry: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for PPM1F

Target: PPM1F (Protein Phosphatase 1F (PP2C Domain Containing) (PPM1F))

Alternative Name: Protein Phosphatase 1F / PPM1F

Background: PPM1F is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase can interact with Rho guanine nucleotide exchange factors (PIX), and thus block the effects of p21-activated kinase 1 (PAK), a protein kinase mediating biological effects downstream of Rho GTPases. Calcium/calmodulin-dependent protein kinase II gamma (CAMK2G/CAMK-II) is found to be one of the substrates of this phosphatase. The overexpression of this phosphatase or CAMK2G has been shown to mediate caspase-dependent apoptosis.Synonyms: Ca(2+)/calmodulin-dependent protein kinase phosphatase, CaM-kinase phosphatase, CaMKPase, KIAA0015, POPX2, Partner of PIX 2, Protein fem-2 homolog

Gene ID: 9647, 9606

UniProt: P49593

Pathways: Positive Regulation of Endopeptidase Activity