PPM1F antibody (AA 1-454)

Catalog No. ABIN5776247

The Mouse Monoclonal anti-PPM1F antibody (Clone AT6D11) (ABIN5776247) specifically detects PPM1F in WB, ELISA, IF and ICC. The antibody is reactive with Human samples.

Quick Overview for PPM1F antibody (AA 1-454) (ABIN5776247)

Target: PPM1F (Protein Phosphatase 1F (PP2C Domain Containing) (PPM1F))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PPM1F antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: AT6D11

Product Details anti-PPM1F Antibody

Binding Specificity: AA 1-454

Purpose: Human PPM1F antibody

Purification: Purified

Immunogen: Recombinant human PPM1F (1-454aa) purified from E. coli

Isotype: IgG1 kappa

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate-Buffered Saline ( pH 7.4) with 0.02 % Sodium Azide, 10 % glycerol

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing cycles

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Target Details for PPM1F

Target: PPM1F (Protein Phosphatase 1F (PP2C Domain Containing) (PPM1F))

Alternative Name: PPM1F

Background: PPM1F is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase can interact with Rho guanine nucleotide exchange factors (PIX), and thus block the effects of p21-activated kinase 1 (PAK), a protein kinase mediating biological effects downstream of Rho GTPases. Calcium/calmodulin-dependent protein kinase II gamma (CAMK2G/CAMK-II) is found to be one of the substrates of this phosphatase. The overexpression of this phosphatase or CAMK2G has been shown to mediate caspase-dependent apoptosis. An alternatively spliced transcript variant has been identified, but its full-length nature has not been determined.

NCBI Accession: NP_055449

Pathways: Positive Regulation of Endopeptidase Activity