Lamin B1 antibody

Catalog No. ABIN360850

This Mouse Monoclonal antibody specifically detects Lamin B1 in WB, IF, FACS, IHC (fro) and EIA. It exhibits reactivity toward Human, Mouse, Rat, Cow, Rabbit, Dog, Sheep and Zebrafish (Danio rerio).

Quick Overview for Lamin B1 antibody (ABIN360850)

Target: Lamin B1 (LMNB1)

Reactivity: Human, Mouse, Rat, Cow, Rabbit, Dog, Sheep, Zebrafish (Danio rerio)

Host: Mouse

Clonality: Monoclonal

Conjugate: This Lamin B1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Clone: 119D5-F1

Product Details anti-Lamin B1 Antibody

Specificity: The antibody Clone 119D5-F1 reacts with an epitope located C-terminal of residue 231 in Lamin B1.

Purification: Purified

Immunogen: Purified Rat liver lamins.

Isotype: IgG1

Application Details

Application Notes: Immunocytochemistry (1: 100 - 1: 200 with avidin-biotinylated horseradish peroxidasecomplex (ABC) as detection reagent). Immunoblotting (1: 100 - 1: 1000). Flow cytometry (1: 100 - 1: 200). ELISA.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Lamin B1

Target: Lamin B1 (LMNB1)

Alternative Name: Lamin-B1 (LMNB1)

Background: Nuclear lamins form a network of intermediate-type filaments at the nucleoplasmic site of the nuclear membrane.Two main subtypes of nuclear lamins can be distinguished, i.e. A-type lamins and B-type lamins. The A-type lamins comprise a set of three proteins arising from the same gene by alternative splicing, i.e. lamin A, lamin C and lamin Adel 10, while the B-type lamins include two proteins arising from two distinct genes, i.e. lamin B1 and lamin B2.Synonyms: LMN2, LMNB, LMNB-1, LMNB1, Lamin-B1, Nuclear Envelope Marker

Molecular Weight: 67 kDa (Predicted)

Gene ID: 4001

NCBI Accession: NP_005564

UniProt: P20700

Pathways: Apoptosis, Caspase Cascade in Apoptosis