Podoplanin antibody (Biotin)

Catalog No. ABIN360862

This Hamster Monoclonal antibody specifically detects Podoplanin in WB, IP, IHC (p) and IHC (fro). It exhibits reactivity toward Mouse.

Quick Overview for Podoplanin antibody (Biotin) (ABIN360862)

Target: Podoplanin (PDPN)

Reactivity: Mouse

Host: Hamster

Clonality: Monoclonal

Conjugate: This Podoplanin antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: RTD4E10

Product Details anti-Podoplanin Antibody (Biotin)

Specificity: The unconjugated antibody will detect Podoplanin on the surface of Mouse lymphatic endothelial cells and some epithelial cell types by Immunostaining or Immunohistochemistry. The antibody does not cross-react with Human Podoplanin.

Purification: Protein G Chromatography

Immunogen: Murine thymic stromal cell line

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry on Frozen and Paraffin Sections: 1-10 μg/mL. Western blot: 1-10 μg/mL (The antibody will detect a 36-40 kDa band). Immunoprecipitation: 1-5 μg IgG per 1 mLlysate or reaction volume.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.4, 0.02 % Sodium Azide, 50 x BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: This product is photosensitive and should be protected from light

Storage: 4 °C

Storage Comment: Prior to and following reconstitution store the antibody undiluted at 2-8 °C. DO NOT FREEZE!

Target Details for Podoplanin

Target: Podoplanin (PDPN)

Alternative Name: Podoplanin

Background: Podoplanin, also known as glycoprotein 36 (gp36), PA2.26 antigen, T1-alpha (T1A), and aggrus, is a 36 kDa type I transmembrane sialoglycoprotein and member of the Podoplanin family. Podoplanin has three potential splice variants, the longest of which is represented by a 238 amino acid precursor. It contains an undefined signal sequence, a 22 aa transmembrane segment (aa 207-228) and a short cytoplasmic tail (aa 229-238). The cytoplasmic tail contains putative sites for protein kinase C phosphorylation. There are two potential alternate start sites at Met 77 (Swiss Prot #: Q86YL7) and Met 119 (EAW51692) that generate short forms. The 162 aa short form Podoplanin precursor shares 47 % aa identity with mouse Podoplanin. Podoplanin is expressed on glomerular epithelial cells (podocytes), type I lung alveolar cells, lymphatic endothelial cells, and numerous tumors, including colorectal tumors, squamous cell carcinomas, testicular seminoma, and brain tumors. One study shows high expression of Podoplanin mRNA in placenta, lung, skeletal muscle, and heart, and weaker levels in brain, kidney, and liver. Podoplanin is the ligand for C-type lectin-like receptor 2 (CLEC-2). Their association is dependent on sialic acid on O-glycans of Podoplanin. Through its association with CLEC-2, Podoplanin induces platelet aggregation and tumor metastasis. Podoplanin is also necessary for lymphatic vessel formation, normal lung cell proliferation and alveolus formation at birth.Synonyms: Aggrus, GP36, Glycoprotein 36, PA2.26 antigen, PDPN, PSEC0003, PSEC0025, T1-alpha

Gene ID: 14726

NCBI Accession: NP_034459

UniProt: Q62011

Pathways: Dicarboxylic Acid Transport