IL3RA antibody (Extracellular Domain)

Catalog No. ABIN360906

This anti-IL3RA antibody is a Rat Monoclonal antibody detecting IL3RA in WB. Suitable for Mouse.

Quick Overview for IL3RA antibody (Extracellular Domain) (ABIN360906)

Target: IL3RA (Interleukin 3 Receptor, alpha (IL3RA))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This IL3RA antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 9G52

Product Details anti-IL3RA Antibody

Binding Specificity: Extracellular Domain

Specificity: This antibody detects IL-3Ralpha. Do not cross react to IL-3Rbeta.

Purification: Protein A/G affinity chromatography

Immunogen: Recombinant protein of IL-3Ralpha extracellular domain.

Isotype: IgG2

Application Details

Application Notes: Western Blot: 1/500 - 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 200 μL sterile PBS and the final concentration is 500 μg/mL.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for IL3RA

Target: IL3RA (Interleukin 3 Receptor, alpha (IL3RA))

Alternative Name: CD123 / IL3RA

Background: IL3RA (CD123) is a member of the immunoglobulin superfamily that is expressed on hematopoietic progenitors, basophils, mast cells, and megakaryocytes. This transmembrane glycoprotein can bind IL3 with low affinity but cannot transduce signals without association with additional protein partners. CD123 can complex with either common beta chain (CDw131) or the IL3RB chain to form high affinity heterodimeric IL3 receptors. CDw131 can complex with the Alpha subunits of the mouse IL3R, IL5R and GMCSFR to form high affinity receptors, while the IL3RB subunit is specific for IL3 but binds with low affinity. IL3 binding to the receptor complex can induce proliferation and differentiation of hematopoietic cells. It is present in Hematopoietic progenitors, basophils, mast cells and megakaryocytes.Synonyms: IL-3R-alpha, IL3 receptor alpha, Interleukin-3 receptor subunit alpha

Gene ID: 16188

NCBI Accession: NP_032395

UniProt: P26952

Pathways: JAK-STAT Signaling