IL-6 Receptor antibody (Extracellular Domain)

Catalog No. ABIN360909

This anti-IL-6 Receptor antibody is a Rat Monoclonal antibody detecting IL-6 Receptor in WB and IHC (fro). Suitable for Mouse.

Quick Overview for IL-6 Receptor antibody (Extracellular Domain) (ABIN360909)

Target: IL-6 Receptor (IL6R) (Interleukin 6 Receptor (IL6R))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This IL-6 Receptor antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 14F33

Product Details anti-IL-6 Receptor Antibody

Binding Specificity: Extracellular Domain

Specificity: This antibody detects Mouse IL-6R. Other species not tested.

Purification: Protein A/G affinity chromatography

Immunogen: Recombinant protein of IL-6R extracellular domain.

Isotype: IgG2

Application Details

Application Notes: Western Blot: 1/500 - 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 0.2 mL sterile PBS and the final concentration is 0.5 mg/mL.

Buffer: PBS, None

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for IL-6 Receptor

Target: IL-6 Receptor (IL6R) (Interleukin 6 Receptor (IL6R))

Alternative Name: CD126 / IL6R

Background: IL6 exerts its activity through binding to a high affinity receptor complex consisting of two membrane glycoproteins: an 80 kDa IL6 binding receptor protein (IL6R) and a 130 kDa signal transducing protein consisting of a 19 amino acid residue signal peptide (gp130). Human IL6R is a 468 amino acid residue type I membrane protein consisting of a 19 amino acid residue signal peptide, a 339 residue extracellular region, a 28 residue transmembrane region and an 82 residue cytoplasmic region. The ectodomain of IL6R is made up of an immunoglobulin like domain and a cytokine receptor superfamily domain. A naturally occurring soluble form of the IL6R, which binds IL6 and which mediates IL6 signaling through interaction with gp130, is identified in the urine of healthy adult humans. It has also been detected in human and mouse serum as well as in the conditioned medium of various cells including human peripheral blood mononuclear cells, and several T cell or granulocyte/macrophage cell lines. Increased expression of the c-fos and c-myc protooncogenes have been linked to IL6 receptor activation.Synonyms: IL-6R 1, IL-6R-alpha, IL6 Receptor alpha, Interleukin-6 receptor subunit alpha, Membrane glycoprotein 80

Gene ID: 16194

NCBI Accession: NP_034689

UniProt: P22272

Pathways: JAK-STAT Signaling, Autophagy, Growth Factor Binding, Cancer Immune Checkpoints