LEFTY1 antibody

Catalog No. ABIN360926

The Rat Monoclonal anti-LEFTY1 antibody is suitable to detect LEFTY1 in samples from Mouse. It has been validated for WB.

Quick Overview for LEFTY1 antibody (ABIN360926)

Target: LEFTY1 (Left-Right Determination Factor 1 (LEFTY1))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This LEFTY1 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 8B65

Product Details anti-LEFTY1 Antibody

Specificity: This antibody detects Lefty-1.

Purification: Protein A/G affinity chromatography

Immunogen: Recombinant protein of Lefty-1

Isotype: IgG2

Application Details

Application Notes: Western Blot: 1/500 - 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 200 μL sterile PBS and the final concentration is 500 μg/mL.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for LEFTY1

Target: LEFTY1 (Left-Right Determination Factor 1 (LEFTY1))

Alternative Name: LEFTY1

Background: During vertebrate embryogenesis, a left right axis is established. Secreted growth factors of the TGF beta family, including gene products derived from nodal, lefty 1 and lefty 2, play crucial roles in establishing left right asymmetries. TGF beta (Transforming growth factor beta) is a pleiotropic cytokine that regulates growth and differentiation of diverse types of cells. TGF beta actions are directed by ligand induced activation of TGF beta receptors. Complexes formed move into the nucleus, where they act as components of a transcriptional complex. Lefty, a novel member of the TGF beta superfamily, inhibits TGF beta signaling. Lefty acts to inhibit phosphorylation of Smad2 following activation of the TGF beta receptor. Lefty also inhibits events downstream from R Smad phosphorylation. Lefty provides a repressed state of TGF beta responsive genes. The Lefty family is comprised of Lefty 1 and Lefty 2 in mouse, and Lefty A and Lefty B in humans. Members of the TGF beta superfamily require processing for their activation. Cleavage is therefore an essential step for Lefty activation. Lefty is synthesized as a large inactive precursor (41 Kda) that must be endoproteolytically processed to release the bioactive polypeptide (28 kDa and 34 kDa forms). The 28 kDa form induces MAPK activity.Synonyms: LEFTB, LEFTY1, LEFTYB, Left-right determination factor 1, Left-right determination factor B, Protein lefty-1, Protein lefty-B

Gene ID: 13590

NCBI Accession: NP_034224

UniProt: Q64280