SPARC antibody

Catalog No. ABIN360936

The Rat Monoclonal anti-SPARC antibody (Clone 5F41) (ABIN360936) specifically detects SPARC in IHC (fro) and EIA. The antibody is reactive with Mouse samples.

Quick Overview for SPARC antibody (ABIN360936)

Target: SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This SPARC antibody is un-conjugated

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Clone: 5F41

Product Details anti-SPARC Antibody

Specificity: This antibody detects SPARC.

Purification: Protein A/G affinity chromatography

Immunogen: Recombinant SPARK

Isotype: IgG2

Application Details

Application Notes: Immunohistochemistry on frozen sections: 1/50 - 1/200. ELISA: 1/400 - 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 200 μL sterile PBS and the final concentration is 500 μg/mL.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for SPARC

Target: SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))

Alternative Name: SPARC / Osteonectin

Background: Synonyms: BM-40, Basement-membrane protein 40, ON, Secreted protein acidic and rich in cysteine

Gene ID: 20692

NCBI Accession: NP_033268

UniProt: P07214

Pathways: Autophagy