TWSG1 antibody

Catalog No. ABIN360945

This anti-TWSG1 antibody is a Rat Monoclonal antibody detecting TWSG1 in WB and IHC (fro). Suitable for Mouse.

Quick Overview for TWSG1 antibody (ABIN360945)

Target: TWSG1 (Twisted Gastrulation Homolog 1 (TWSG1))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This TWSG1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 7D19

Product Details anti-TWSG1 Antibody

Specificity: This antibody detects Mouse TSG. Other species not tested.

Purification: Protein A/G Affinity Chromatography

Immunogen: Purified Mouse Recombinant TSG

Isotype: IgG2

Application Details

Application Notes: Western Blot: 1/500 - 1/1000. Immunohistochemistry on frozen sections: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 200 μL sterile PBS and the final concentration is 500 μL/mL.

Buffer: PBS, None

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for TWSG1

Target: TWSG1 (Twisted Gastrulation Homolog 1 (TWSG1))

Alternative Name: TWSG1 / Tsg

Background: Twisted gastrulation (TSG) is a secreted, cysteine-rich protein that plays a role in dorsal/ventral patterning in Drosophila and Xenopus by regulating BMP signaling. TSG was originally identified in Drosophila melanogaster and shown to be required for the differentiation of the dorsal amnioserosa cells. Studies of expression and function of Twisted Gastrulation have been performed in Drosophila and Xenopus. Xenopus TSG is expressed in the ventral regions of the embryo during gastrulation, mimicking the BMP-4 expression pattern. TSG is expressed in dorsal cells of the blastoderm embryo, where there are also high levels of activity of Dpp and Screw. Vertebrate TSGs were subsequently cloned in mouse, human, zebrafish and frog. TSG acts as an agonist for BMP signaling by modulating the inhibitory actions of the BMP antagonist, Chordin/Sog, and the cleavage properties of the metalloprotease, xolloid/tolloid. The N-terminal domain of TSG can bind BMP protein directly in vitro and shows BMP antagonist activity.Synonyms: Twisted gastrulation protein homolog 1

Gene ID: 65960

NCBI Accession: NP_075540

UniProt: Q9EP52