XPNPEP1 antibody

Catalog No. ABIN360952

This Mouse Monoclonal antibody specifically detects XPNPEP1 in IF, IHC (p) and IHC (fro). It exhibits reactivity toward Rat.

Quick Overview for XPNPEP1 antibody (ABIN360952)

Target: XPNPEP1 (X-Prolyl Aminopeptidase (Aminopeptidase P) 1, Soluble (XPNPEP1))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This XPNPEP1 antibody is un-conjugated

Application: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: JG12C9C10

Product Details anti-XPNPEP1 Antibody

Specificity: This antibody reacts to Aminopeptidase P.

Purification: Protein G chromatography

Immunogen: BALB/c mice using a membrane protein fraction of isolated rat glomeruli

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry on frozen and paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL. Centrifuge vial prior to opening.

Buffer: PBS pH 6,0

Precaution of Use: Use of sodium azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for XPNPEP1

Target: XPNPEP1 (X-Prolyl Aminopeptidase (Aminopeptidase P) 1, Soluble (XPNPEP1))

Alternative Name: XPNPEP1

Background: Aminopeptidase P (AP-P, X-Pro aminopeptidase) has the unique ability to leave the N-terminal amino acid residue from peptides having proline as the penultimate amino acid residue. Biologically active peptides comprise an important and diverse class of extracellular chemical messengers that mediate a wide range of intercellular interactions. Several bioactive peptides including hormones, neuropeptides, neurotransmitters escape non-specific protease degradation by having an Xaa-Pro motif at their amino termini. Due to its cyclic nature, proline confers resistance to such peptide bonds so that aminopeptidases with broad specificity cannot act upon such peptides. There are a limited number of peptidases that act on peptide bonds involving a proline residue, such as dipeptidyl peptidase II (DPPII) and dipeptidyl peptidase W (DPPIV), and prolidase (which cleaves the Xaa-Pro bond only in dipeptides), or endopeptidases such as prolyl endopeptidase (which cleaves on the carbonyl side of proline residues within a protein or peptide). However, none of these enzymes have been reported to hydrolyze Xaa-Pro bonds located at the N-terminus of peptides and proteins. Therefore, role of AP-P is crucial in this respect. AP-P activity is ubiquitous and has been found in a wide range of organisms including bacteria, yeast and vertebrates. Mammalian AP-Ps exist in membrane-bound and cytosolic forms, which represent two distinct gene products. The cytosolic (soluble) form of aminopeptidase P is found in human leukocytes and rat brain.Synonyms: Aminoacylproline aminopeptidase, Cytosolic aminopeptidase P, Soluble aminopeptidase P, X-Pro aminopeptidase 1, X-prolyl aminopeptidase 1 soluble, XPNPEPL, XPNPEPL1, Xaa-Pro aminopeptidase 1

Gene ID: 7511

NCBI Accession: NP_065116

UniProt: Q9NQW7