SCARB2 antibody (Internal Region)

Catalog No. ABIN361195

This Goat Polyclonal antibody specifically detects SCARB2 in WB, ELISA and IHC. It exhibits reactivity toward Humanand has been mentioned in 1 publication.

Quick Overview for SCARB2 antibody (Internal Region) (ABIN361195)

Target: SCARB2 (Scavenger Receptor Class B, Member 2 (SCARB2))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This SCARB2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-SCARB2 Antibody

Binding Specificity: Internal Region

Purpose: LIMP2 / SCARB2

Sequence: NKANIQFGDN GTTIS

Specificity: This antibody is expected to recognize reported isoform 1 (NP_005497.1) only.

Cross-Reactivity: Human, Mouse

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-NKANIQFGDNGTTIS, from the internal region of the protein sequence according to NP_005497.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Cerebral Cortex shows lysosomal staining. Recommended concentration, 3-5 μg/mL.

Western Blot: Approx. 80 kDa band observed in Human Brain (Cortex and Cerebellum) lysates (calculated MW of 54.3 kDa according to NP_005497.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Fujita et

Peptide ELISA: antibody detection limit dilution 1:4000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-SCARB2 antibody (ABIN361195)

Murphy, Gysbers, Abbott, Tayebi, Kim, Sidransky, Cooper, Garner, Halliday: "Reduced glucocerebrosidase is associated with increased ?-synuclein in sporadic Parkinson's disease." in: Brain : a journal of neurology, Vol. 137, Issue Pt 3, pp. 834-48, (2014) (PubMed).

Target Details for SCARB2

Target: SCARB2 (Scavenger Receptor Class B, Member 2 (SCARB2))

Alternative Name: SCARB2

Background: SCARB2, scavenger receptor class B, member 2, AMRF, CD36L2, HLGP85, LIMPII, 85 kDa lysosomal sialoglycoprotein scavenger receptor class B, member 2, CD36 antigen (collagen type I receptor, thrombospondin receptor)-like 2 (lysosomal integral membrane prote

Gene ID: 950, 12492

NCBI Accession: NP_005497