3,5-Dibromo-Tyrosine antibody

Catalog No. ABIN361274

The Mouse Monoclonal anti-3,5-Dibromo-Tyrosine antibody is suitable to detect 3,5-Dibromo-Tyrosine. It has been validated for IHC, ELISA and WB.

Quick Overview for 3,5-Dibromo-Tyrosine antibody (ABIN361274)

Target: 3,5-Dibromo-Tyrosine

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Western Blotting (WB)

Clone: 3A5

Product Details

Specificity: This antibody can react with dibromotyrosine (DiBrY), N-acetylated dichloro tyrosine (NAcDiClY), diiodo tyrosine (DiIY), N-bromosuccinimide-treated BSA, HOCl-treated BSA and dibromo-tyrosone conjugated BSA (halogene-modified tyrosine at both 3- and 5-position). Cross reactivity to following analogues have been tested: dityrosine-conjugated BSA, peroxynitrite-treated BSA, tyrosine (Y), chrolotyrosine(ClY), 3-3

Isotype: IgG1 kappa

Application Details

Application Notes: Immunohistochemistry (Recommended concentration: 10ug/mL IgG) [ref.1] and western blotting.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Target Details

Target: 3,5-Dibromo-Tyrosine

Target Type: Amino Acid

Background: Tyrosine is one of the main targets of protein oxidation, and it have been reported form nitrotyrosine, dityrosine and halogenated tyrosines depending on the type of free radicals. For example, hypochlorous acid (HOCl) and hypobromous acid (HOBr) are derived from neutrophil myeloperoxidase (MPO) and eosinophil peroxidase (EPO), and known to form dihalogenated tyrosines such as DiBrY, DiClY and DiIY. It is expected that DiBrY may be a novel biomarker for tissue damage which is related to inflammatory and allergic disorders.