Periostin antibody (C-Term)

Catalog No. ABIN361361

The Rabbit Polyclonal anti-Periostin antibody has been validated for WB and IHC. It is suitable to detect Periostin in samples from Mouse.

Quick Overview for Periostin antibody (C-Term) (ABIN361361)

Target: Periostin (POSTN)

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Periostin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Periostin Antibody

Binding Specificity: C-Term

Specificity: Specific for the ~93 kDa periostin doublet in mouse lung extract. This antibody does not recognize the single heart form of periostin or the lowest molecular weight form of lung periostin. Consistent with the fact that this antibody is made against an alternatively spliced region of periostin, this antibody recognizes a subset of the forms of periostin recognized on Western blots by the Pan periostin antibody (Catalog number 1622-PERI) and also shows a distinctive staining pattern by immunohistochemistry (data not shown). The antibody works well for immunohistochemistry on paraformaldehyde-fixed sections with a simple antigen-retrieval protocol (incubate slides for 20 minutes at 90° C in 10 mM sodium citrate (pH 6.0)/ 0.1 % Tween-20). The antibody is expected to work on all mammalian , avian species.

Cross-Reactivity: Chicken, Human, Mouse (Murine)

Predicted Reactivity: most mammalian and avian

Purification: Antigen Affinity Purified

Immunogen: Bacterial fusion protein equivalent to a 188-amino acid polypeptide from the C-terminal region of mouse periostin which is comprised of six small alternatively-spliced exons

Application Details

Application Notes: Recommended Dilution: WB: 1:1,000 IHC: 1:100 Quality Control: Western blots performed on each lot.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 μL in PBS

Storage: -20 °C

Target Details for Periostin

Target: Periostin (POSTN)

Alternative Name: POSTN

Background: Periostin is a matricellular protein, i.e. an extracellular matrix protein that interacts both with other ECM proteins and with cell-surface receptors. Like many other matricellular proteins, the function of periostin is important both in embryonic development and in the remodeling of adult tissues in response to pathological insults. Periostin was originally isolated as an osteoblast-specific marker that functions as a cell adhesion molecule for preosteoblasts and is thought to be involved in osteoblast recruitment, attachment and spreading (Kruzynska-Frejtag A. et al., 2004). Periostin has recently been shown to promote collagen fibrogenesis, inhibit differentiation of progenitor cells into cardiomyocytes and to be essential in maintaining the biomechanical properties of the adult myocardium (Norris et al., 2008) Anti-Periostin Western blot of rat lung lysate showing specific immunolabeling of the ~ 93 kDa periostin protein doublet.

Molecular Weight: '93 kDa

Gene ID: 50706

UniProt: Q62009