Peripherin antibody

Catalog No. ABIN361363

This Mouse Monoclonal antibody specifically detects Peripherin in WB and IF. It exhibits reactivity toward Rat.

Quick Overview for Peripherin antibody (ABIN361363)

Target: Peripherin (PRPH)

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Peripherin antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: 7C5

Product Details anti-Peripherin Antibody

Specificity: Specific for the ~57kDa peripherin protein. This antibody performs well on aldehyde fixed tissues.

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Predicted Reactivity: most mammalian

Purification: Protein G purified culture supernatant

Immunogen: Recombinant rat peripherin expressed in and purified from E. coli

Isotype: IgG1

Application Details

Application Notes: Recommended Dilution: WB:1:1,000 IF: 1:200 Quality Control: Western blots performed on each lot.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: total IgG fraction + 10 mM Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Target Details for Peripherin

Target: Peripherin (PRPH)

Alternative Name: PRPH

Background: Peripherin is a ~57kDa intermediate filament subunit found initially in sensory neurons of the peripheral nervous systems, which gives the protein its name. Subsequently, peripherin was found in some sensory and other neurons of the central nervous system and also in PC12 cells. Peripherin is also expressed in certain neuroendocrine tumors and in the insulin producing cells of the pancreas. Peripherin belongs to the Class III family of intermediate filament subunits which also includes vimentin, glial fibrillary acidic protein (GFAP) and desmin. In contrast to the neurofilaments, peripherin is strongly up-regulated after nerve injury (1). Antibodies to peripherin can be used in identifying, classifying, and studying neurons throughout the nervous system. Peripherin is also a good diagnostic marker for ballooned axons seen in Lou Gehrig's disease (Amyotrophic lateral sclerosis) and some neuronally derived tumors (2). Autoantibodies to peripherin are frequently seen in the sera of patients with diabetes (3). Peripherin is not related to peripherin/RDS, a protein of the photoreceptor outer membrane mutations of which are causative of certain forms of slow retinal degeneration. Anti-Peripherin Western blot of rat cerebellar lysate showing specific immunolabeling of the ~ 57k peripherin protein.

Molecular Weight: '57 kDa

Gene ID: 3414

UniProt: P21807