Rho-related GTP-binding protein antibody

Catalog No. ABIN361366

This Mouse Monoclonal antibody specifically detects Rho-related GTP-binding protein in WB and IHC. It exhibits reactivity toward Cow and has been mentioned in 2+ publications.

Quick Overview for Rho-related GTP-binding protein antibody (ABIN361366)

Target: Rho-related GTP-binding protein (RhO (pan))

Reactivity: Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This Rho-related GTP-binding protein antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: 1D4

Product Details anti-Rho-related GTP-binding protein Antibody

Specificity: Specific for the ~ 39k rhodopsin protein.

Cross-Reactivity: Amphibian, Mammalian

Purification: Protein G purified culture supernatant

Immunogen: Purified native bovine rhodopsin

Isotype: IgG1

Application Details

Application Notes: Recommended Dilution: WB: 1:1000 IHC: 1:100 Quality Control: Western blots performed on each lot.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 μL in 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Storage: -20 °C

References for anti-Rho-related GTP-binding protein antibody (ABIN361366)

Cho, Song, Shin, Kim: "Neonatal disease environment limits the efficacy of retinal transplantation in the LCA8 mouse model." in: BMC ophthalmology, Vol. 16, Issue 1, pp. 193, (2017) (PubMed).

Kim, Park, Lee, Shin, Nickas, Kim, Cho: "Yap is essential for retinal progenitor cell cycle progression and RPE cell fate acquisition in the developing mouse eye." in: Developmental biology, Vol. 419, Issue 2, pp. 336-347, (2017) (PubMed).

Target Details for Rho-related GTP-binding protein

Target: Rho-related GTP-binding protein (RhO (pan))

Alternative Name: RHO

Target Type: Chemical

Background: Rhodopsin is a photoreceptor protein found in retinal rods. It is a complex formed by the binding of retinaldehyde, the oxidized form of retinol, to the protein opsin and undergoes a series of complex reactions in response to visible light resulting in the transmission of nerve impulses to the brain. Mutation of the rhodopsin gene is a major contributor to various retinopathies such as retinitis pigmentosa. The disease-causing protein generally aggregates with ubiquitin in inclusion bodies, disrupts the intermediate filament network and impairs the ability of the cell to degrade non-functioning proteins which leads to photoreceptor apoptosis (Berson et al., 1991). Other mutations on rhodopsin lead to X-linked congenital stationary night blindness, mainly due to constitutive activation, when the mutations occur around the chromophore binding pocket of rhodopsin (Dryja et al.,1993). Several other pathological states relating to rhodopsin have been discovered including poor post-Golgi trafficking, dysregulative activation, rod outer segment instability and arrestin binding. Anti-Rhodopsin Immunohistochemical staining of mouse retinal section showing specific immunolabeling of the rhodopsin protein in the rod spherules. Photo courtesy of Mary Raven, University of California, Santa Barbara, CA.

Molecular Weight: '39 kDa

Gene ID: 509933

UniProt: P02699