GABRA1 antibody (N-Term)

Catalog No. ABIN361435

This Rabbit Polyclonal antibody specifically detects GABRA1 in WB. It exhibits reactivity toward Ratand has been mentioned in 1 publication.

Quick Overview for GABRA1 antibody (N-Term) (ABIN361435)

Target: GABRA1 (gamma-aminobutyric Acid (GABA) A Receptor, alpha 1 (GABRA1))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GABRA1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-GABRA1 Antibody

Binding Specificity: N-Term

Specificity: Specific for the ~51k a1-subunit of the GABAA receptor in Western blots of rat brain. Labeling is absent in alpha1-subunit knockout animals.

Cross-Reactivity: Mouse (Murine), Rat (Rattus)

Predicted Reactivity: bovine, canine, human, non-human primate

Purification: Antigen Affinity Purified

Immunogen: Synthetic peptide corresponding to amino acid residues from the N-terminal region conjugated to KLH

Application Details

Application Notes: Recommended Dilution: WB: 1:1000 Quality Control: Western blots performed on each lot.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 μL in 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Storage: -20 °C

References for anti-GABRA1 antibody (ABIN361435)

Agoglia, Zhu, Ying, Sidhu, Natividad, Wolfe, Buczynski, Contet, Parsons, Roberto, Herman: "Corticotropin-Releasing Factor Receptor-1 Neurons in the Lateral Amygdala Display Selective Sensitivity to Acute and Chronic Ethanol Exposure." in: eNeuro, Vol. 7, Issue 2, (2020) (PubMed).

Target Details for GABRA1

Target: GABRA1 (gamma-aminobutyric Acid (GABA) A Receptor, alpha 1 (GABRA1))

Alternative Name: GABRA1

Background: Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl- channel associated with the GABAA receptor (GABAA-R) subtype. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABAA-R is a multimeric subunit complex. To date six (s, four (s and four (s, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990, Whiting et al., 1999, Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for (- and (-subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a (-subunit is required for benzo-diazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different (-subunits of the receptor (McKernan et al., 2000, Mehta and Ticku, 1998, Ogris et al., 2004, Pöltl et al., 2003). Anti-GABAA Receptor, a1-Subunit Western blot of mouse forebrain lysates from Wild Type (Control) and a1-knockout (a1-K/O) animals showing specific immunolabeling of the ~51k a1-subunit of the GABAA-R. The labeling was absent from a lysate prepared from a1-knockout animals.

Molecular Weight: '51 kDa

Gene ID: 29705

UniProt: P62813