gamma-aminobutyric Acid (GABA) A Receptor, beta 2 (GABRB2) (Cytoplasmic Loop) antibody

Details for Product No. ABIN361447
Target
Binding Specificity
Cytoplasmic Loop
10
8
7
7
4
3
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Rat
63
41
37
9
8
7
5
5
5
4
4
4
3
3
2
1
1
Host
Rabbit
60
7
Clonality
Polyclonal
Conjugate
Un-conjugated
6
5
5
3
3
3
2
1
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
41
31
13
10
6
1
1
1
1
Options
Immunogen Fusion protein from the cytoplasmic loop of the beta 2 subunit
Specificity Specific for the ~55k ß2-subunit of the GABAA receptor in Western blots.
Cross-Reactivity Mouse (Murine), Rat (Rattus)
Predicted Reactivity canine, human, non-human primate
Purification Antigen Affinity Purified
Target
Alternative Name GABRB2 (GABRB2 Antibody Abstract)
Background Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl- channel associated with the GABAA receptor (GABAA-R) subtype. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABAA-R is a multimeric subunit complex. To date six (s, four (s and four (s, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990, Whiting et al., 1999, Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for (- and (-subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a (-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different (-subunits of the receptor (McKernan et al., 2000, Mehta and Ticku, 1998, Ogris et al., 2004, Pöltl et al., 2003). Anti-GABAA-Receptor, (2-Subunit Western blot of 7 (g of rat cerebellum (Cb) showing specific immunolabeling of the ~55k ß2-subunit of the GABAA-R.
Molecular Weight '55 kDa
Gene ID 25451
UniProt P63138
Pathways Sensory Perception of Sound, Synaptic Membrane
Application Notes Recommended Dilution: WB: 1:1000 Quality Control: Western blots performed on each lot.
Restrictions For Research Use only
Format Liquid
Buffer 100 μL in 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.
Storage -20 °C
Images
Image no. 1 for anti-gamma-aminobutyric Acid (GABA) A Receptor, beta 2 (GABRB2) (Cytoplasmic Loop) antibody (ABIN361447) Western blots of 7 (g of rat cerebellum (Cb) showing specific immunolabeling of the ~...
Product cited in: Antflick, Hampson: "Modulation of glutamate release from parallel fibers by mGlu4 and pre-synaptic GABA(A) receptors." in: Journal of neurochemistry, Vol. 120, Issue 4, pp. 552-63, 2012 (PubMed). (1:500).