DARPP32 antibody (pThr34)

Catalog No. ABIN361462

This Rabbit Polyclonal antibody specifically detects DARPP32 in WB and ELISA. It exhibits reactivity toward Rat and has been mentioned in 8+ publications.

Quick Overview for DARPP32 antibody (pThr34) (ABIN361462)

Target: DARPP32 (PPP1R1B) (Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DARPP32 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-DARPP32 Antibody

Binding Specificity: pThr34

Specificity: Specific for endogenous levels of the ~32 kDa DARPP-32 protein phosphorylated at Thr34. Immunolabeling is completely eliminated by treatment with λ-phosphatase.

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: bovine, canine, chicken, human, non-human primates

Characteristics: Our DARPP-32 (Thr34) rabbit polyclonal phosphospecific primary antibody is produced in-house. It detects DARPP-32 (Thr34) and is antigen affinity purified from pooled serum. It is great for use in ELISA, WB.

Purification: Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr34 of rat DARPP-32, conjugated to keyhole limpet hemocyanin (KLH).

Isotype: IgG

Application Details

Application Notes:

Dilution Range: WB Brain: 1:1000

Dilution Range: WB: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Storage: -20 °C

Storage Comment: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Expiry Date: 12 months

References for anti-DARPP32 antibody (ABIN361462)

DelGuidice, Latapy, Rampino, Khlghatyan, Lemasson, Gelao, Quarto, Rizzo, Barbeau, Lamarre, Bertolino, Blasi, Beaulieu: "FXR1P is a GSK3β substrate regulating mood and emotion processing." in: Proceedings of the National Academy of Sciences of the United States of America, (2015) (PubMed).

Gomez, Midde, Mactutus, Booze, Zhu: "Environmental enrichment alters nicotine-mediated locomotor sensitization and phosphorylation of DARPP-32 and CREB in rat prefrontal cortex." in: PLoS ONE, Vol. 7, Issue 8, pp. e44149, (2013) (PubMed).

Marazziti, Di Pietro, Mandillo, Golini, Matteoni, Tocchini-Valentini: "Absence of the GPR37/PAEL receptor impairs striatal Akt and ERK2 phosphorylation, DeltaFosB expression, and conditioned place preference to amphetamine and cocaine." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 25, Issue 6, pp. 2071-81, (2011) (PubMed).

Daigle, Wetsel, Caron: "Opposite function of dopamine D1 and N-methyl-D-aspartate receptors in striatal cannabinoid-mediated signaling." in: The European journal of neuroscience, Vol. 34, Issue 9, pp. 1378-89, (2011) (PubMed).

Napolitano, Bonito-Oliva, Federici, Carta, Errico, Magara, Martella, Nistica: "Role of aberrant striatal dopamine D1 receptor/cAMP/protein kinase A/DARPP32 signaling in the paradoxical calming effect of amphetamine." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 30, Issue 33, pp. 11043-56, (2010) (PubMed).

Santini, Sgambato-Faure, Li, Savasta, Dovero, Fisone, Bezard: "Distinct changes in cAMP and extracellular signal-regulated protein kinase signalling in L-DOPA-induced dyskinesia." in: PLoS ONE, Vol. 5, Issue 8, pp. e12322, (2010) (PubMed).

Niculescu, Perrine, Miller, Ehrlich, Unterwald: "Trk: a neuromodulator of age-specific behavioral and neurochemical responses to cocaine in mice." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 28, Issue 5, pp. 1198-207, (2008) (PubMed).

Beaulieu, Sotnikova, Gainetdinov, Caron: "Paradoxical striatal cellular signaling responses to psychostimulants in hyperactive mice." in: The Journal of biological chemistry, Vol. 281, Issue 43, pp. 32072-80, (2006) (PubMed).

Target Details for DARPP32

Target: DARPP32 (PPP1R1B) (Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B))

Alternative Name: PPP1R1B

Background:

Synonyms: DARPP32 antibody, DARPP-32 antibody, Dopamine and cAMP regulated neuronal phosphoprotein 32 antibody, Dopamine and cAMP regulated neuronal phosphoprotein antibody, Dopamine and cAMP regulated phosphoprotein antibody, Dopamine and cAMP regulated phosphoprotein DARPP 32 antibody, Dopamine and cAMP regulated phosphoprotein DARPP32 antibody, Dopamine- and cAMP-regulated neuronal phosphoprotein antibody, FLJ20940 antibody, IPPD antibody, Neuronal phosphoprotein DARPP 32 antibody, PPP1R1B antibody, PPR1B_HUMAN antibody, Protein phosphatase 1 regulatory (inhibitor) subunit 1B antibody, Protein phosphatase 1 regulatory inhibitor subunit 1B antibody, Protein phosphatase 1 regulatory subunit 1B antibody

Description Logic: Our DARPP-32 (Thr34) rabbit polyclonal phosphospecific primary antibody is produced . It detects DARPP-32 (Thr34) and is antigen affinity purified from pooled serum. It is great for use in ELISA, WB.

Gene Name Alternatives: PPP1R1B

Molecular Weight: 32 kDa

Gene ID: 360616

UniProt: Q6J4I0