AQP2 antibody (pSer261)

Catalog No. ABIN361469

There are 2+ publications for this product available. The Rabbit Polyclonal anti-AQP2 antibody is suitable to detect AQP2 in samples from Rat. It has been validated for WB, IHC, ELISA, IP and ICC.

Quick Overview for AQP2 antibody (pSer261) (ABIN361469)

Target: AQP2 (Aquaporin 2 (Collecting Duct) (AQP2))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AQP2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-AQP2 Antibody

Binding Specificity: pSer261

Specificity: Specific for endogenous levels of the ~29 kDa AQP2 protein phosphorylated at Ser261. Also recognizes the glycosylated form of AQP2 at ~37 kDa. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: bovine, canine, chicken, human, non-human primate

Characteristics: Our Aquaporin 2 (Ser261) rabbit polyclonal phosphospecific primary antibody is produced in-house. It detects Aquaporin 2 (Ser261) and is antigen affinity purified from pooled serum. It is great for use in ELISA, WB, IHC, ICC, IP.

Purification: Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser261 of rat aquarporin 2, conjugated to keyhole limpet hemocyanin (KLH).

Isotype: IgG

Application Details

Application Notes:

Dilution Range: IP: 3 µL

Dilution Range: ICC: 1:500-1:3000

Dilution Range: WB: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Storage: -20 °C

Storage Comment: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Expiry Date: 12 months

References for anti-AQP2 antibody (ABIN361469)

Hoffert, Fenton, Moeller, Simons, Tchapyjnikov, McDill, Yu, Pisitkun, Chen, Knepper: "Vasopressin-stimulated increase in phosphorylation at Ser269 potentiates plasma membrane retention of aquaporin-2." in: The Journal of biological chemistry, Vol. 283, Issue 36, pp. 24617-27, (2008) (PubMed).

Hoffert, Nielsen, Yu, Pisitkun, Schleicher, Nielsen, Knepper: "Dynamics of aquaporin-2 serine-261 phosphorylation in response to short-term vasopressin treatment in collecting duct." in: American journal of physiology. Renal physiology, Vol. 292, Issue 2, pp. F691-700, (2007) (PubMed).

Target Details for AQP2

Target: AQP2 (Aquaporin 2 (Collecting Duct) (AQP2))

Alternative Name: AQP2

Background:

Synonyms: ADH water channel antibody, AQP 2 antibody, AQP CD antibody, AQP-2 antibody, AQP-CD antibody, AQP2 antibody, AQP2_HUMAN antibody, AQPCD antibody, Aquaporin 2 collecting duct antibody, Aquaporin CD antibody, Aquaporin-2 antibody, Aquaporin-CD antibody, Aquaporin2 antibody, Aquaporine 2 antibody, Collecting duct water channel protein antibody, MGC34501 antibody, Water channel aquaporin 2 antibody, Water channel protein for renal collecting duct antibody, WCH CD antibody, WCH-CD antibody, WCHCD antibody

Description Logic: Our Aquaporin 2 (Ser261) rabbit polyclonal phosphospecific primary antibody is produced . It detects Aquaporin 2 (Ser261) and is antigen affinity purified from pooled serum. It is great for use in ELISA, WB, IHC, ICC, IP.

Gene Name Alternatives: AQP2

Molecular Weight: 29 kDa/37 kDa

Gene ID: 25386

UniProt: P34080

Pathways: Response to Water Deprivation