STXBP2 antibody (pSer513)

Catalog No. ABIN361480

This Rabbit Polyclonal antibody specifically detects STXBP2 in WB and IHC. It exhibits reactivity toward Rat.

Quick Overview for STXBP2 antibody (pSer513) (ABIN361480)

Target: STXBP2 (Syntaxin Binding Protein 2 (STXBP2))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This STXBP2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-STXBP2 Antibody

Binding Specificity: pSer513

Specificity: Specific for the ~65k Munc-18 protein phosphorylated at Ser515. Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide.

Cross-Reactivity: Rat (Rattus)

Predicted Reactivity: bovine, canine, chicken, human, mouse, non-human primate, Xenopus

Purification: Antigen Affinity Purified from Pooled Serum

Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser513 conjugated to KLH

Application Details

Application Notes: Recommended Dilution: WB: 1:1000 IHC: 1:400 Quality Control: Western blots performed on each lot.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 μL in 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Storage: -20 °C

Target Details for STXBP2

Target: STXBP2 (Syntaxin Binding Protein 2 (STXBP2))

Alternative Name: STXBP2

Background: Munc-18 ( mammalian homologue of Unc-18) is a protein that is thought to be involved in regulating exocytosis due, at least in part, to it ability to bind syntaxin (Ciufo et al., 2005). Munc18-1 is a neuron-specific member of the Sec1/Munc18 protein family that binds to syntaxin1A and is thought to stabilize the complex (Liu et al., 2004). The function of Munc-18 is thought to be regulated by PKC phosphorylation of Ser515 on the Munc-18 protein (Sassa et al., 1996). Anti-Phospho-Ser515 Munc-18 Western blot of rat cortex lysate showing specific immunolabeling of the ~65k Munc-18 protein phosphorylated at Ser515. Immunolabeling is blocked by the phosphopeptide (peptide) used as antigen but not by the corresponding dephosphopeptide (not shown). .

Molecular Weight: '65 kDa

Gene ID: 81804

UniProt: Q62753

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Synaptic Vesicle Exocytosis