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GRIN2B antibody (pSer1480)

GRIN2B Reactivity: Rat WB Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN361486
  • Target See all GRIN2B Antibodies
    GRIN2B (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2B (GRIN2B))
    Binding Specificity
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    pSer1480
    Reactivity
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    Rat
    Host
    • 185
    • 37
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    Rabbit
    Clonality
    • 187
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    Polyclonal
    Conjugate
    • 101
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    This GRIN2B antibody is un-conjugated
    Application
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    Western Blotting (WB)
    Specificity
    Specific for ~180k NMDAR NR2B subunit protein phosphorylated at Ser1480. Immunolabeling of the NMDA NR2B subunit band is blocked by the phosphopeptide used as the antigen but not by the corresponding dephosphopeptide. Immunolabeling is also blocked by (-phosphatase treatment.
    Cross-Reactivity
    Mouse (Murine), Rat (Rattus)
    Predicted Reactivity
    bovine, canine, chicken, human, non-human primate, Xenopus
    Purification
    Antigen Affinity Purified from Pooled Serum
    Immunogen
    Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser1480 conjugated to KLH
    Top Product
    Discover our top product GRIN2B Primary Antibody
  • Application Notes
    Recommended Dilution: WB: 1:1000 Quality Control: Western blots performed on each lot.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 μL in 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.
    Storage
    -20 °C
  • Target
    GRIN2B (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2B (GRIN2B))
    Alternative Name
    GRIN2B (GRIN2B Products)
    Background
    The NMDA receptor (NMDAR) plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002, Wenthold et al., 2003, Carroll and Zukin, 2002). The rat NMDAR1 (NR1) was the first subunit of the NMDAR to be cloned. The NR1 protein can form NMDA activated channels when expressed in Xenopus oocytes but the currents in such channels are much smaller than those seen in situ. Channels with more physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits (Ishii et al., 1993). It has been shown that phosphorylation of Ser1480 disrupts the interaction of NR2B with the PDZ domains of PSD-95 and SAP102 and decreases surface NR2B expression in neurons (Chung et al., 2004). Anti-Phospho-Ser1480 NMDA Receptor NR2B Subunit Western blot of rat hippocampal lysate showing specific immunolabeling of the ~180k NR2B subunit of the NMDAR phosphorylated at Ser1480 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: (-Ptase). The blot is identical to the control except that it was incubated in (-Ptase (1200 units for 30 min) before being exposed to the phospho-Ser1480 NMDA NR2B subunit antibody. The immunolabeling is completely eliminated by treatment with (-Ptase.
    Molecular Weight
    '180 kDa
    Gene ID
    24410
    UniProt
    Q00960
    Pathways
    Response to Growth Hormone Stimulus, Synaptic Membrane, Feeding Behaviour, Regulation of long-term Neuronal Synaptic Plasticity
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