RPH3A antibody (pSer234)

Catalog No. ABIN361489

This Rabbit Polyclonal antibody specifically detects RPH3A in WB, ELISA and ICC. It exhibits reactivity toward Ratand has been mentioned in 1 publication.

Quick Overview for RPH3A antibody (pSer234) (ABIN361489)

Target: RPH3A (Rabphilin 3A (RPH3A))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RPH3A antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC)

Product Details anti-RPH3A Antibody

Binding Specificity: pSer234

Specificity: Specific for endogenous levels of the ~82 kDa rabphilin protein phosphorylated at Ser234. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.

Cross-Reactivity: Rat

Predicted Reactivity: mouse

Characteristics: Our Rabphilin 3A (Ser234) rabbit polyclonal phosphospecific primary antibody is produced in-house. It detects Rabphilin 3A (Ser234) and is antigen affinity purified from pooled serum. It is great for use in ELISA, WB, ICC.

Purification: Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser234 of rat rabphilin 3A, conjugated to keyhole limpet hemocyanin (KLH).

Isotype: IgG

Application Details

Application Notes:

Dilution Range: ICC: 1:400

Dilution Range: WB Brain: 1:1000

Dilution Range: WB: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Storage: -20 °C

Storage Comment: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Expiry Date: 12 months

References for anti-RPH3A antibody (ABIN361489)

Stephens, Powell, Taraska, Harris: "Imaging the rapid yet transient accumulation of regulatory lipids, lipid kinases, and protein kinases during membrane fusion, at sites of exocytosis of MMP-9 in MCF-7 cells." in: Lipids in health and disease, Vol. 19, Issue 1, pp. 195, (2020) (PubMed).

Target Details for RPH3A

Target: RPH3A (Rabphilin 3A (RPH3A))

Alternative Name: RPH3A

Background:

Synonyms: Exophilin 1 antibody, Exophilin-1 antibody, Exophilin1 antibody, KIAA0985 antibody, Rabphilin 3A homolog antibody, Rabphilin-3A antibody, Rabphilin3A antibody, RP3A antibody, RP3A_HUMAN antibody, Rph 3a antibody, Rph3a antibody

Description Logic: Our Rabphilin 3A (Ser234) rabbit polyclonal phosphospecific primary antibody is produced . It detects Rabphilin 3A (Ser234) and is antigen affinity purified from pooled serum. It is great for use in ELISA, WB, ICC.

Gene Name Alternatives: RPH3A

Molecular Weight: 82 kDa

Gene ID: 171039

UniProt: P47709

Pathways: Synaptic Vesicle Exocytosis