GRP94 antibody

Catalog No. ABIN361654

This anti-GRP94 antibody is a Rat Monoclonal antibody detecting GRP94 in WB, FACS, IF, IP and ICC. Suitable for Chicken.

Quick Overview for GRP94 antibody (ABIN361654)

Target: GRP94 (HSP90B1) (Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1))

Reactivity: Chicken

Host: Rat

Clonality: Monoclonal

Conjugate: This GRP94 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: 9G10

Product Details anti-GRP94 Antibody

Specificity: Detects ~98 kDa. Does not detect human HSP90, Grp74, or GrpE from E.coli.

Cross-Reactivity: Chicken, Cow, Dog, Guinea Pig, Hamster, Horse, Human, Monkey, Mouse, Pig, Rabbit, Rat, Sheep, Xenopus laevis

Purification: Protein G Purified

Immunogen: Purified Grp94 isolated from chicken oviducts

Isotype: IgG2a

Application Details

Application Notes:

• WB (1:2000)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Comment:

0.5 μg/ml of ABIN361653 was sufficient for detection of Grp94 in 20 μg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-rat IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.2, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: -20°C

Target Details for GRP94

Target: GRP94 (HSP90B1) (Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1))

Alternative Name: GRP94

Background: Grp94 (glucose regulated protein 94, gp96) is a constitutively expressed endoplasmic reticulum (ER) lumenal protein that is up-regulated in response to cellular stress such as heat shock, oxidative stress or glucose depletion. Grp94 is thought to play a role in protein translocation to the ER, in their subsequent folding and assembly, and in regulating protein secretion (1). Grp94 also plays a role in antigen presentation by accessing the endogenous pathway and eliciting specific CTL responses to chaperone bound peptides via MHC class I pathway (2). Grp94 is a member of the HSP90 family of stress proteins and shares sequence homology with its cytosolic equivalent, HSP90 (3). Both HSP90 and Grp94 are calcium binding proteins (4). Despite sharing 50 % sequence homology over its N domains and complete conservation in its ligand binding domains with HSP90, Grp94 and HSP90 differ in their interactions with regulatory ligands as Grp94 has weak ATP binding and hydrolysis activity (5). Grp94 exists as a homodimer and the two subunits interact at two distinct intermolecular sites, C terminal dimerization domains and the N-terminal interacts with the middle domain of opposing subunits (6). Grp94 contains a carboxy terminal KDEL (Lys-Asp-Glu-Leu) sequence which is believed to aid in its retention in the ER (7).

Gene ID: 7184

NCBI Accession: NP_003290

UniProt: P14625

Pathways: Thyroid Hormone Synthesis, Activation of Innate immune Response, ER-Nucleus Signaling, Toll-Like Receptors Cascades