Phosphoserine antibody

Catalog No. ABIN361725

This anti-Phosphoserine antibody is a Rabbit Polyclonal antibody detecting Phosphoserine in WB, ELISA, IP, IHC, ICC and IF. Suitable for . This Primary Antibody has been cited in 2+ publications.

Quick Overview for Phosphoserine antibody (ABIN361725)

Target: Phosphoserine

Reactivity: Please inquire

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Phosphoserine antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-Phosphoserine Antibody

Specificity: Detects proteins phosphorylated on serine residues. Does not cross-react with phosphotyrosine.

Purification: Peptide Affinity Purified

Immunogen: Phosphoserine conjugated to KLH, and phosvitin mixture

Application Details

Application Notes:

• WB (1:500)
• ICC/IF (1:50)
• ELISA (1:250)
• IP (1:100)
• optimal dilutions for assays should be determined by the user.

Comment:

2 μg/ml of ABIN361725 was sufficient for detection of phosphorylation signal in western blot analysis using human MMRU cells treated with 0.1 μM okadaic acid.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS, 50 % glycerol, 0.01 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: -20°C

References for anti-Phosphoserine antibody (ABIN361725)

Shalem-Cohavi, Beery, Nordenberg, Rozovski, Raanani, Lahav, Uziel: "The Effects of Proteasome Inhibitors on Telomerase Activity and Regulation in Multiple Myeloma Cells." in: International journal of molecular sciences, Vol. 20, Issue 10, (2019) (PubMed).

Uziel, Cohen, Beery, Nordenberg, Lahav: "The effect of Bortezomib and Rapamycin on Telomerase Activity in Mantle Cell Lymphoma." in: Translational oncology, Vol. 7, Issue 6, pp. 741-51, (2014) (PubMed).

Target Details for Phosphoserine

Target: Phosphoserine

Target Type: Amino Acid

Background: Protein phosphorylation is an important posttranslational modification that serves many key functions to regulate a protein's activity, localization, and protein-protein interactions. Phosphorylation is catalyzed by various specific protein kinases, which involves removing a phosphate group from ATP and covalently attaching it to to a recipient protein that acts as a substrate. Most kinases act on both serine and threonine, others act on tyrosine, and a number (dual specificity kinases) act on all three. Because phosphorylation can occur at multiple sites on any given protein, it can therefore change the function or localization of that protein at any time (1). Changing the function of these proteins has been linked to a number of diseases, including cancer, diabetes, heart disease, inflammation and neurological disorders (2-4).