SYT2 antibody (pThr202)

Catalog No. ABIN362244

The Rabbit Polyclonal anti-SYT2 antibody is suitable to detect SYT2 in samples from Human, Rat and Mouse. It has been validated for IF.

Quick Overview for SYT2 antibody (pThr202) (ABIN362244)

Target: SYT2 (Synaptotagmin II (SYT2))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SYT2 antibody is un-conjugated

Application: Immunofluorescence (IF)

Product Details anti-SYT2 Antibody

Binding Specificity: pThr202

Specificity: The antibody detects endogenous level of Synaptotagmin II only when phosphorylated at threonine202.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: Peptide sequence around phosphorylation site of pThr202 (R-K-T (p) -L-N) derived from Human. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotype: IgG

Application Details

Application Notes: Immunofluorescence: 1:100-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Target Details for SYT2

Target: SYT2 (Synaptotagmin II (SYT2))

Alternative Name: Synaptotagmin II

Background: The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin I participates in triggering neurotransmitter release at the synapse

Molecular Weight: 46 kDa

NCBI Accession: NP_001129

UniProt: Q8N9I0

Pathways: Synaptic Vesicle Exocytosis