The antibody detects endogenous level of total MyoD protein.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Immunogen
Peptide sequence around AA 198-202 (A-S-S-P-R) derived from Human MyoD. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.
MYOD1
Reactivity: Human
ELISA
Host: Mouse
Monoclonal
1C8
unconjugated
Application Notes
Western blotting: 1:500-1:1000
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C/-20 °C
Storage Comment
Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Target
MYOD1
(Myogenic Differentiation 1 (MYOD1))
Alternative Name
MyoD
Background
MyoD encodes a nuclear protein that belongs to the basic helix-loop-helix family of transcription factors and the myogenic factors subfamily. It regulates muscle cell differentiation by inducing cell cycle arrest, a prerequisite for myogenic initiation. The protein is also involved in muscle regeneration. It activates its own transcription which may stabilize commitment to myogenesis.