SMC1A antibody (AA 955-959)

Catalog No. ABIN362824

This anti-SMC1A antibody is a Rabbit Polyclonal antibody detecting SMC1A in WB and IHC. Suitable for Human and Mouse.

Quick Overview for SMC1A antibody (AA 955-959) (ABIN362824)

Target: SMC1A (Structural Maintenance of Chromosomes 1A (SMC1A))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMC1A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-SMC1A Antibody

Binding Specificity: AA 955-959

Specificity: The antibody detects endogenous level of total SMC1 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Peptide sequence around AA 955-959 (G-S-S-Q-G) derived from Human SMC1. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.

Isotype: IgG

Application Details

Application Notes: Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Target Details for SMC1A

Target: SMC1A (Structural Maintenance of Chromosomes 1A (SMC1A))

Alternative Name: SMC1

Background: Involved in chromosome cohesion during cell cycle and in DNA repair. Central component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Involved in DNA repair via its interaction with BRCA1 and its related phosphorylation by ATM, or via its phosphorylation by ATR. Works as a downstream effector both in the ATM/NBS1 branch and in the ATR/MSH2 branch of S-phase checkpoint.

Molecular Weight: 145 kDa

NCBI Accession: NP_006297

UniProt: Q14683

Pathways: Stem Cell Maintenance