Ephrin B2 antibody (AA 328-332)

Catalog No. ABIN362834

This Rabbit Polyclonal antibody specifically detects Ephrin B2 in WB and IF. It exhibits reactivity toward Human, Mouse and Ratand has been mentioned in 1 publication.

Quick Overview for Ephrin B2 antibody (AA 328-332) (ABIN362834)

Target: Ephrin B2 (EFNB2)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Ephrin B2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-Ephrin B2 Antibody

Binding Specificity: AA 328-332

Specificity: The antibody detects endogenous level of total Ephrin-B2 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific immunogen.

Immunogen: Peptide sequence around AA 328-332 (N-I-Y-Y-K) derived from Human Ephrin B (ephrin-B2) . Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.

Isotype: IgG

Application Details

Application Notes: Western blotting: 1:500-1:1000
Immunofluorescence: 1:100-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

References for anti-Ephrin B2 antibody (ABIN362834)

Toribio, Brown, Novince, Marlow, Hernon, Lanigan, Hildreth, Werbeck, Shu, Lorch, Carlton, Foley, Boyaka, McCauley, Rosol: "The midregion, nuclear localization sequence, and C terminus of PTHrP regulate skeletal development, hematopoiesis, and survival in mice." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 24, Issue 6, pp. 1947-57, (2010) (PubMed).

Target Details for Ephrin B2

Target: Ephrin B2 (EFNB2)

Alternative Name: Ephrin-B2

Background: Ephrin-B2 encodes a member of the ephrin (EPH) family. The ephrins and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, especially in the nervous system and in erythropoiesis. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a lycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. This gene encodes an EFNB class ephrin which binds to the EPHB4 and EPHA3 receptors.

Molecular Weight: 37 kDa

NCBI Accession: NP_004084

UniProt: P52799

Pathways: RTK Signaling, Regulation of Muscle Cell Differentiation