EIF4E antibody (AA 207-211)

Catalog No. ABIN362892

The Rabbit Polyclonal anti-EIF4E antibody has been validated for WB, IHC and IF. It is suitable to detect EIF4E in samples from Human, Rat and Mouse.

Quick Overview for EIF4E antibody (AA 207-211) (ABIN362892)

Target: EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF4E antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-EIF4E Antibody

Binding Specificity: AA 207-211

Specificity: The antibody detects endogenous level of total elF4E protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific immunogen.

Immunogen: Peptide sequence around AA 207-211 (S-G-S-T-T) derived from Human eIF4E. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.

Isotype: IgG

Application Details

Application Notes: Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Immunofluorescence: 1:100-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Target Details for EIF4E

Target: EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))

Alternative Name: eIF4E

Background: Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures.

Molecular Weight: 25 kDa

NCBI Accession: NP_001124

UniProt: P06730

Pathways: BCR Signaling